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Consistent with its disruptions of mitochondrial respiration reflected in the cells’ OCR measurements, CB-839, starting at low concentrations of 0.1 to 1 μM, also decreased the ATP pool in the HG-3 (P = 0.02) and MEC-1 (P = 0.01) cell lines, as measured using high-performance liquid chromatography ( Figure 2D ).The levels of other nucleotides remained stable at all concentration points